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The Nrf2 independent regulation of GSTP1 expression in HaCaT cells might have selectively evolved with high proliferation capacity during immortalization. The average luciferase activity of HaCaT ARE reporter cells treated by ginger phenylpropanoids and quercetin was greater than that of their controls by 4. These results are also consistent with previous studies that showed the effects of ginger phenylpropanoids and quercetin in the protection against oxidative stress in human skin cells [ 20 — 22 ]. Both, ginger phenylpropanoids and quercetin, significantly increased the level of Nrf2 activity. At the end of the incubation period, the cells were assayed using MTT. Clones were selected and validated according to instructions provided by manufacturer.

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Such a discrepancy between the increased level of Nrf2 and its downstream effectors in HaCaT cells was also reported in previous studies of Zhang et al.

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At the end of the incubation period, the cells were assayed using MTT. The average level of this protein did not differ significantly neither between the HaCaT cells treated by ginger phenylpropanoids and quercetin and that of their controls values for effects of ginger phenylpropanoids and quercetin were and 0. On the other hand, while both ginger phenylpropanoids and quercetin can activate Nrf2 in HaCaT cells, their effects on expression of the GSTP1 were not mediated.

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